Bath Rugby's Kyle Eastmond, who made his Test debut in last Saturday's 32-3 win in Salta, will start for the first time in the No.12 shirt vacated by Lions-bound centre Billy Twelvetrees (Gloucester Rugby).
And fly half Stephen Myler (Northampton Saints) will win a first cap if he comes off the replacements' bench.
England are bidding to secure a first series win in Argentina since 1981, when Bill Beaumont's tourists drew the first and won the second of their two Tests.
Head Coach Stuart Lancaster said: "It is great to reward young players who have pushed through this season. They have worked hard to earn their opportunities on this tour.
"This is a young England side which made a very positive start to the series in Salta. But we expect Argentina to come back very strongly in front of a big home crowd. It's a great challenge and one we are all looking forward to."
15 Mike Brown (Harlequins, 17 caps)
14 Christian Wade (Wasps, 1 cap)
13 Jonathan Joseph (London Irish, 5 caps)
12 Kyle Eastmond (Bath Rugby, 1 cap)
11 Marland Yarde (London Irish, uncapped)
10 Freddie Burns (Gloucester Rugby, 2 caps)
16 David Paice (London Irish, 7 caps)
17 Paul Doran Jones (Northampton Saints, 5 caps)
18 Henry Thomas (Sale Sharks, 1 cap)
19 Courtney Lawes (Northampton Saints, 21 caps)
20 Billy Vunipola (London Wasps, 1 cap)
21 Richard Wigglesworth (Saracens, 13 caps)
22 Stephen Myler (Northampton Saints, uncapped)
23 Ben Foden (Northampton Saints, 31 caps)