The 36-player squad will meet at Pennyhill Park next week for a Guinness Six Nations training camp.
There is a first call up for Northampton Saints’ Fin Smith. The fly half is joined by four other uncapped players – Ollie Hassell-Collins, George McGuigan, Cadan Murley and Jack Walker.
Courtney Lawes returns to the squad following injury. There are also returns for forwards Dan Cole, Ben Curry, Alex Dombrandt and Nick Isiekwe, as well as backs Elliot Daly, Dan Kelly, Max Malins and Joe Marchant.
England’s Six Nations fixtures
“This is an exciting squad, with a blend of Six Nations experience and young talent, and includes players who are in excellent form in the Premiership,” said Borthwick.
“We are all looking forward to the challenges of the Six Nations and we will approach this great tournament with a spirit of courage and total commitment. I know the players can’t wait to get back to Twickenham and give our fans a performance they can be proud of.
“The hard work for the Scotland game starts now.”
George McGuigan (Gloucester Rugby, uncapped)
Jack Walker (Harlequins, uncapped)
Ollie Hassell-Collins (London Irish, uncapped)
Cadan Murley (Harlequins, uncapped)
Fin Smith (Northampton Saints, uncapped)
Jack van Poortvliet